ABSTRACT
Oral Lichen Planus [OLP] is a chronic immunologic disorder with unknown etiology. Stress and anxiety are some risk factors for OLP and Vitamin B12 and folic acid have been proved to be effective mirco-nutrients for prevention of anxiety and depression. The purpose of this study was to investigate serum Vitamin B12 and folic acid deficiency in patients with OLP. Forty eight individuals [32 cases with histopathologically proven OLP and 16 healthy subjects] were recruited. Serum folic acid and Vitamin B12 were assessed for each individual. T-test was applied for data analysis. Vitamin B12 deficiency was found in 8 of 32 cases with OLP [25%] while in the control group it was found in 12.5% of the subjects [p>0.05]. Of the 32 patients with OLP, one had folic acid deficiency while none of the control subjects had such a deficiency [p>0.05]. Although Vitamin B12 deficiency in OLP patients did not show a statistically significant difference compared with healthy subjects, it was approximately twice as much as the control group. And it can be suggested that Vitamin B12 may have some effective roles in OLP pathogenesis while folic acid deficiency cannot be considered as a prominent risk factor in OLP. More studies are needed to prove such relation
Subject(s)
Humans , Male , Female , Vitamin B 12/blood , Folic Acid/blood , Case-Control StudiesABSTRACT
Great interest has recently been focused on the addition of herbs and their derivatives to foods and biological system, due to their well-known abilities to scavenge free radicals. The aims of this study were to evaluate the antioxidant activity of the essential oil of Zataria multiflora Boiss. and [ii] to use this essential oil as natural antioxidant in crude soy bean oil. In this study, essential oil was: [i] analyzed by GC/MS and its chemical components were identified. The antioxidant activity of essential oil was investigated with DPPH? free radical scavenging and beta-carotene bleaching methods and was compared with synthetic antioxidant [BHT]. Also, the antioxidant activity of essential oil was determined by measuring peroxide and thiobarbituric acid values in crude soy bean oil [oven test]. Results showed that carvacrol [26.08%] and thymol [17.23%] were main phenolic components in the essential oil. The EC50 value of Zataria multiflora essential oil was determined as 2.22 +/- 0.04 mg/ml. This parameter for BHT was 0.58 +/- 0.02. By the beta-carotene bleaching system, antioxidant activity of essential oil at 0.45% was equal to 72% and for BHT at 0.1% was determined 81%. In oven test, the antioxidant activity of Zataria multiflora essential oil at 0.1% was almost equal to BHA at 0.02% in crude soy bean oil. The essential oil showed good antioxidant activity and therefore, it can be used as a natural antioxidant in some foods after complementary tests